Please reposition the bottom of the column holder after Loosen the screw.
- The system flow rate is too high for the current flash column.
- Sample has poor solubility and precipitates from the mobile phase, thus resulting tubing blockage.
- Other reason causes tubing blockage.
The environment is too wet, or the solvent leakage to the inside of the column holder causes short circuit. Please heat the column holder properly by a hair dryer or a hot air gun after power off.
Solvent leakage might be due to the solvent level in the waste bottle is higher than the height of the connector at the base of the column holder.
Place the waste bottle below the operation platform of the instrument, or quickly move down the column holder after removing the column.
This cleaning function is designed to clean the system pipeline before separation run. If “post-cleaning” has been performed after the last separation run, this step could be skipped. If it is not performed, it is recommended to do this cleaning step as instructed by the system prompt.
No, end-capped silica is insoluble in any commonly used organic solvent.
For optimal purification with C18 flash columns, please follow these steps:
- Flush the column with 100% of the strong (organic) solvent for 10 – 20 CVs (column volume), typically methanol or acetonitrile.
- Flush the column with 50% strong + 50% aqueous (if additives are required, include them) for another 3 – 5 CVs.
- Flush the column with the initial gradient conditions for 3 – 5 CVs.
For column size between 4g and 330g, standard Luer connector is used in these flash columns. For column size of 800g, 1600g and 3000g, additional connector adaptors should be used to mount these large flash columns on the flash chromatography system. Please refer to the document Santai Adaptor Kit for 800g, 1600g, 3kg Flash Columns for more details.
For normal phase column, it is recommended to use the mobile phase where the ratio of methanol does not exceed 25%.
